The band filed the complaint within hours of the video going live.
Queen have taken on President Donald Trump and won, with a campaign video featuring their song We Will Rock You in its entirety being removed from his Twitter following a copyright complaint from the band’s publisher.
Last week, Trump posted a campaign video that featured the iconic song and, according to Buzzfeed, within hours Queen had "already entered into a process to call for non use of Queen song copyrights by the Trump campaign”.
After being viewed more than 1.7 million times, the video was disabled by Twitter on Sunday and the post now reads: "This media has been disabled in response to a report by copyright owner.”
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Last week, Prince’s estate called out Trump for playing Purple Rain at a campaign event, despite previously agreeing they would not use his music.
Earlier this year, Trump was threatened with legal action for using parts of the score from The Dark Knight Rises in a video on Twitter, while Pharrell Williams, Steven Tyler, REM and more have previously protested the use of their songs at Trump events.